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Identification of uncommon plant metabolites based on ...

Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry Anal Chem . 2000 Aug 1;72(15):3573-80. doi: 10.1021/ac991142i.

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Identification of Uncommon Plant Metabolites Based on ...

Jul 07, 2000  Identification of Uncommon Plant Metabolites Based on Calculation of Elemental Compositions Using Gas Chromatography and Quadrupole Mass Spectrometry. Oliver Fiehn, Joachim Kopka, Richard N. Trethewey, and ; Lothar Willmitzer

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Identification of Uncommon Plant Metabolites Based on ...

Jul 07, 2000  Unknown compounds in polar fractions of Arabidopsis thaliana crude leaf extracts were identified on the basis of calculations of elemental compositions obtained from ...

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Identification of Uncommon Plant Metabolites Based on ...

Identification of Uncommon Plant Metabolites Based on Calculation of Elemental Compositions Using Gas Chromatography and Quadrupole Mass Spectrometry Oliver Fiehn,* Joachim Kopka,† Richard N. Trethewey,† and Lothar Willmitzer Max-Planck-Institute of Molecular Plant Physiology, 14424 Potsdam, Germany Unknown compounds in polar fractions of ...

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Identification of uncommon plant metabolites based on ...

T1 - Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. AU - Fiehn, Oliver. AU - Kopka, Joachim. AU - Trethewey, Richard N. AU - Willmitzer, Lothar. PY - 2000/8/1. Y1 - 2000/8/1

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Identification of uncommon plant metabolites based on ...

Aug 01, 2000  Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. O Fiehn Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany.

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Identification of uncommon plant metabolites based on ...

T1 - Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. AU - Fiehn, Oliver. AU - Kopka, Joachim. AU - Trethewey, Richard N. AU - Willmitzer, Lothar. PY - 2000/8/1. Y1 - 2000/8/1

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Identification of uncommon plant metabolites based on ...

More than 15 uncommon plant metabolites were identified, some of which are novel in Arabidopsis, such as tartronate semialdehyde, citramalic acid, allothreonine, or glycolic amide. ISSN : 0003-2700 DOI : 10.1021/ac991142i

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Identification of Uncommon Plant Metabolites Based on ...

Identification of Uncommon Plant Metabolites Based on Calculation of Elemental Compositions Using Gas Chromatography and Quadrupole Mass Spectrometry September 2000

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Identification of uncommon plant metabolites based on ...

Autor: Fiehn, O. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2000; Keywords: urinary organic-acids electrospray-ionization sugar alcohols amino-acids gc-ms derivatives recognition; Titel: Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry

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Identification of metabolites related to mechanisms of ...

Identification of metabolites related to mechanisms of resistance in barley against Fusarium graminearum, based on mass spectrometry Plant Mol Biol . 2011 Nov;77(4-5):355-70. doi: 10.1007/s11103-011-9815-8.

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Metabolomics - Plant Molecular Biology

Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal. Chem. 72: 3573–3580.

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Scientific Protocols - A simple protocol for extraction ...

Dec 13, 2012  Quantify each component based on peak areas and normalization based on the internal standard. Timing. ... J., Trethewey, R.N. Willmitzer, L. Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal. Chem.72, 3575–3580 (2000).

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Identification of metabolites from liquid chromatography ...

Liquid chromatography-coulometric array detection (LC-EC) is a sensitive, quantitative, and robust metabolomics profiling tool that complements the commonly used mass spectrometry (MS) and nuclear magnetic resonance (NMR)-based approaches. However, LC-EC provides little structural information. We recently demonstrated a workflow for the structural characterization of metabolites detected by

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Gas chromatography mass spectrometry–based metabolite ...

Jun 27, 2006  GC-MS facilitates the identification and robust quantification of a few hundred metabolites in a single plant extract 7,8,12, resulting in fairly comprehensive coverage of

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Identification of key taste components in loquat using ...

Sep 01, 2020  Thus, to understand the contributions of different metabolite classes, there remains a need for a coherent investigation that report large-scale identification and quantification of metabolites. Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based widely targeted metabolome analysis is a rapid and reliable approach for detection of a ...

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Application of UPLC-QTOF-MS Based Untargeted Metabolomics ...

Metabolomics is a useful tool for comparing metabolite changes in plants. Because of its high sensitivity, metabolomics combined with high-resolution mass spectrometry (HR-MS) is the most widely accepted metabolomics tools. In this study, we compared the metabolites of pathogen-infected rice (Oryza sativa) with control rice using an untargeted metabolomics approach.

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Establishment of retention index library on gas ...

Feb 28, 2013  Fiehn O, Kopka J, Trethewey RN, and Willmitzer L (2000b) Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal Chem 72, 3573–3580. Article CAS Google Scholar

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Metabolomics should be deployed in the identification and ...

Jan 10, 2020  Clearly metabolite identification via species‐specific quantitative data and chromatographic annotation needs addressing. In addition, pre‐purification on the basis of polarity and the development of spatial metabolomics would help to overcome the issue of dynamic range and incorporate more biological texture to the data.

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‪Oliver Fiehn‬ - ‪Google Scholar‬

Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry O Fiehn, J Kopka, RN Trethewey, L Willmitzer Analytical chemistry 72 (15), 3573-3580 , 2000

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Metabolite profiling for plant functional genomics ...

Absolute values were determined for 11 metabolites based on isotope-labeled internal calibration curves. ... Trethewey, R.N. Willmitzer, L. Identification of uncommon plant metabolites based on ...

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Establishment of retention index library on gas ...

Feb 28, 2013  Fiehn O, Kopka J, Trethewey RN, and Willmitzer L (2000b) Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal Chem 72, 3573–3580. Article CAS Google Scholar

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Secondary Metabolites from Plants

Plant Secondary Metabolites • Plants produce as amazing diversity of low molecular weight compounds. • Of the estimated 400,000 – 500,000 plant species around the globe, only a small percentage has been investigated phytochemically and the fraction subjected to biological or pharmacological screening is even lower. Plant Secondary Metabolites

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Figure 1 from Metabolite profiling of germinating rice ...

Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. O. Fiehn , J. Kopka , R. Trethewey ,

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Effects of moist cold stratification on germination, plant ...

Sep 01, 2015  Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry Anal. Chem. , 72 ( 2000 ) , pp. 3573 - 3580 View Record in Scopus Google Scholar

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A simple protocol for extraction, derivatization, and ...

Dec 13, 2012  Quantify each component based on peak areas and normalization based on the internal standard. Timing. ... J., Trethewey, R.N. Willmitzer, L. Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal. Chem.72, 3575–3580 (2000).

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General principle of primary and secondary plant ...

Jan 01, 2021  Processing, extraction, and isolation of plant metabolites. The processes involved in the extraction, isolation, and identification of plant secondary metabolites were summarized. 1.5.1. Selection of specific species of plant. For the investigation of plants for medicinal effects, a common methodology is undertaken.

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Metabolomics should be deployed in the identification and ...

Jan 10, 2020  Clearly metabolite identification via species‐specific quantitative data and chromatographic annotation needs addressing. In addition, pre‐purification on the basis of polarity and the development of spatial metabolomics would help to overcome the issue of dynamic range and incorporate more biological texture to the data.

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MS/MS spectral tag‐based annotation of non‐targeted ...

Identification of unusual plant constituents by interpretation of the MS2T data may reveal the existence of a pathway and the genes responsible for such biosynthesis in plants. Additionally, metabolic phenotyping of a loss-of-function mutant could provide an understanding of

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Plant metabolomics - SlideShare

Jul 24, 2019  Plant metabolomics 1. PLANT METABOLOMICS By, Dr. Rudresh Gowda B B.V.Sc and A.H 2. What is Plant Metabolomics..? • Quantitative and Qualitative analysis, elucidation of all metabolites in Plants under specific conditions • Metabolome-Entire complement of small molecules in plant, Ultimate phenotype of cells, Information of gene expression and modulation of protein function,

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Identification of metabolites from liquid chromatography ...

Liquid chromatography-coulometric array detection (LC-EC) is a sensitive, quantitative, and robust metabolomics profiling tool that complements the commonly used mass spectrometry (MS) and nuclear magnetic resonance (NMR)-based approaches. However, LC-EC provides little structural information. We recently demonstrated a workflow for the structural characterization of metabolites detected by

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Identification and biosynthesis of acylphloroglucinols in ...

Species of the genus Hypericum contain a rich array of unusual polyketides, however, only a small proportion of the over 450 Hypericum species, other than the popular medicinal supplement St. John's Wort (Hypericum perforatum), have even been chemically characterized. Hypericum gentianoides, a

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(PDF) Metabolite Identification in NMR-based Metabolomics ...

Metabolite Identification in NMR-based Metabolomics. 11 Pages. Metabolite Identification in NMR-based Metabolomics. Current Metabolomics, 2015. Santosh K. Bharti. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37

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GC–MS libraries for the rapid identification of ...

Jan 28, 2005  A laboratory which maintains a GC–MS based metabolite profiling facility will continually need to identify metabolites. Frequently, the identity of previously non-identified MSTs will be discovered and the question will arise if these MSTs were found in previous experiments [ 7 , 16 , 22 , 23 ] or by other laboratories [ 8 , 9 ] .

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